报告题目：Constructing Biomimetic Electron Transport Chains for Enzymatic H2 Production and CO2 Fixation in vitro. 

       报 告 人：Eui-Jin Kim博士  弗吉利亚理工大学

　　报告时间：6月15日（周四） 10:00-11:30　　 

　　报告地点：中国科学院天津工业生物技术研究所C408会议室　　 

　　报告摘要：Diaphorase (DI) is an enzyme capable of transferring electrons between NAD(P)H and chemical electron mediators such as benzyl viologen (BV). Biomimetic electron transport chains, comprised of an NADH-dependent or NADPH-dependent DI and a suitable electron mediator, were designed to enhance the electron transfer rates by lowering activation energy barriers. The H₂ generation rates via the biomimetic chains increased by approximately five-fold compared to those catalyzed by [NiFe]-hydrogenase only. We demonstrated an in vitro enzymatic pathway that can produce H₂ at extremely high rates by splitting water energized by starch in the stoichiometric reaction C₆H₁₀O₅ + 7H₂O -> 12H₂ + 6CO₂. This pathway of fifteen hyperthermophilic enzymes is comprised of ATP-free starch phosphorylation, an NAD- rather than NADP-based pentose phosphate pathway, a BV-conjugated DI, and a [NiFe]-hydrogenase. The highest reaction rate of 530 mmole H₂/L/h (i.e., more than 11 L of H₂ per L of bioreactor per h) was accomplished at 80°C. The concept of biomimetic electron transport chains could also be applied to biosystems for artificial photosynthesis. A plausible application scheme of the biomimetic chains for CO₂ fixation will be elucidated.　　

　　报告人简介：Eui-Jin Kim博士分别于2001年、2010年于Sogang University获得理学学士学位和博士学位，并于2010年3月-8月在Sogang University 做博士后研究。2010年9月-2015年7月在Sogang University作研究员，2012年5月-2015年4月作为项目负责人获得由韩国国家研究基金会(NRF)资助的研究项目，研究叶绿素/细菌叶绿素反应机理合成酶，确定底物特异性和抑制剂相互作用的决定因素，取得了重大进展。2015年8月至今，在美国弗吉尼亚理工大学做助理研究员，主要研究领域是利用电子传递链在体外多酶体系的应用来进行高效的氢气生产。在Chemistry，J. Microbiol. Biotechnol,J. Bacterial等杂志上发表了多篇论文，并申请专利10余项。