报告题目：莱茵衣藻三酰基甘油代谢的研究以及功能基因组学工具的研发

　　报 告 人：李小波 博士 （美国斯坦福大学卡内基研究所项目主管）　　 　　 

　　报告时间：11月28日（周五）9:15-10:30　　 

　　报告地点：天津工业生物所 C408 报告厅　　   

　　报告摘要：　　 

　　Microalgae produce large amounts of triacylglycerols (TAGs) under nitrogen deprivation, which can be used as biodiesel feedstocks. Genes required for TAG synthesis are promising targets to be engineered towards enhanced biofuel production. To identify such genes, together with colleagues at Michigan State University, I performed a high-throughput screen for mutants with abnormal TAG content in the model green alga-Chlamydomonas reinhardtii. One mutant, pgd1, exhibited 50% lower TAG content compared to the wild-type. Mechanistic studies suggest that PGD1 functions as a galactoglycerolipid lipase that facilitates export of fatty acids from the chloroplast. Over-expression of PGD1 to enhance TAG production is in progress.　　 

　　At Carnegie Institution for Science, we (the mutant library team in the Jonikas Lab) aim to generate the first genome-wide indexed insertional mutant library for Chlamydomonas. We developed genomic tools for mapping mutation sites as well as robotic approaches for mutant generation, propagation and cryopreservation. We have generated a pilot scale mutant library that covers 8% Chlamydomonas genes with high-confidence. A 10X larger scale library has been generated and is being mapped. This resource will transform genetic studies in microalgae.　　 

　　Besides genetic interrogations of TAG synthesis pathways, we are also interested in understanding the physiological significance of TAG production. Preliminary evidence suggests a role of TAG to serve as an electron sink under nitrogen deprivation to prevent oxidative burst since pgd1 cells die during nitrogen deprivation due to redox imbalance. We developed a tool for pooled screen to obtain all mutants that cannot survive nitrogen deprivation. This will shed light on how photosynthetic organism survive nutrient deprivation as well as providing more TAG deficient mutants.　　 

　　报告人简介：　　 

　　2003-2007， 西安交通大学生物工程系，本科　　 

　　2007-2012， 密歇根州立大学，美国能源部植物研究实验室 （DOE-Plant Research Lab），Christoph Benning 实验室，博士，从事藻类的脂类代谢研究　　 

　　2012至今， 卡内基研究所植物学系，Martin Jonikas 实验室， 项目主管（Project Leader），从事藻类的脂类代谢研究以及基因组学工具的研发。